Abstract

Aim: To prepare BSA loaded chitosan nanoparticles by polymerization technique and to study the effect of initiator concentration upon particle size, product yield, entrapment efficiency, loading capacity and drug release from the formulation.

Methodology: In the present study BSA loaded chitosan nanoparticles were prepared by polymerization technique. Three formulations were prepared by varying the concentration of Initiator. The concentration of Initiator (Ammonium per sulphate) was maintained 1%, 2% 3% in formulation 1, Formulation 2 and Formulation 3 respectively. The effect of initiator concentration on Mean particle diameter, Drug content, entrapment efficiency, loading capacity, electrophoretic mobility and zeta potential was studied.

Results and discussion: Best nanoformulations were obtained with Ammonium per sulphate 2 % concentration with Mean particle diameter of 441.7 nm. Electrophoretic mobility and Zeta potential value (-3.304 and -42.1) was also more among all chitosan formulations indicating greater stability.

Conclusion: Hence Formulation 2 was considered to be the best Formulation for the preparation of BSA loaded Chitosan nanoparticles.